Insulin resistance plays a central role in the pathogenesis of type 2 diabetes mellitus. Ameliorating insulin resistance helps to improve glycaemic control and hence reduce the risk of development and progression of diabetic complications. The extract of Moringa oleifera has been shown to improve hyperglycaemia, however, little is known on its effect on insulin resistance and beta-cell function. The objective of this study was to determine the effect of the ethanolic leaf extract of Moringa oleifera on insulin resistance in high-fat diet and streptozotocin induced diabetic rats. Different doses (250 and 500 mg/kg) of the ethanolic leaf extract of Moringa oleifera were administered orally for two weeks, to high-fat diet and streptozotocin induced diabetic rats, and were compared with metformin 320 mg/kg and control group (distilled water 10 ml/kg) for the effect on fasting blood glucose, serum insulin and insulin resistance (HOMA). The extract of Moringa oleifera (at doses of 250 mg/kg and 500 mg/kg) significantly lowered the fasting blood glucose at days 7 and 14 compared to controls (p = 0.0021 and p = 0.0001 respectively). There was a significant increase in serum insulin level in the control group at days 7 and 14 compared to the groups treated with the extract (250 mg/kg, 500 mg/kg) and metformin (p<0.01, p<0.02 and p<0.01 respectively). The extract at both doses and metformin (320 mg/kg) induced a significant improvement in insulin resistance (HOMA-IR) on days 7 and 14 (p < 0.0001).
| Published in |
Journal of Plant Sciences (Volume 2, Issue 6-1)
This article belongs to the Special Issue Pharmacological and Biological Investigation of Medicinal Plants |
| DOI | 10.11648/j.jps.s.2014020601.12 |
| Page(s) | 5-12 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Insulin Resistance, Moringa oleifera, Diabetes Mellitus, High-Fat Diet
| [1] | Adewunmi, C.O. and J.A.O. Ojewole. (2004). Safety of traditional medicines complementary and alternatives medicines in Africa. Afr. J. Trad. CAM, 1: 1-3. |
| [2] | Ahmed, F and Urooj, (2010). A.Glucose lowering, hepatoprotective and hypolipidemic activities of stem bark of Ficus racemosa in streptozotocin – induced diabetic rats, J young pharm vol (1) ;2 160- 164 |
| [3] | Akah, P.A. and C.L. Okafor. (1992). Phytother. Res.,6:171-173. |
| [4] | Aliyu, R., A.H. Adebayo, D. Gatsing and I.H. Garba. (2007). The effects of ethanolic leaf extract of Commiphora africana (Burseraceae) on rat liver and kidney functions. J. Pharmacol. Toxicol., 2: 373-379. |
| [5] | Amaglo, N.K., Bennett, R.N., LoCurto, R.B., Rosa, E.A.S., LoTurco, V., Giuffrid,A., LoCurto, A., Crea, F., and Timpo, G.M. (2010). Profiling selected phytochemicals and nutrients in different tissues of the multi purpose tree Moringa oleifera L., grownin Ghana. Food Chem. 122, 1047–1054. |
| [6] | Argal, A. and Pathak, A. (2006). KCNS activity of Calotropis gigantean roots. J. Ethnopharmacoogyl., 106: 142-145 |
| [7] | Aslan M, Deliorman Orhan D, Orhan N, Sezik E, Yesilada E (2007). In vivo antidiabetic and antioxidant potential of Helichrysum plicatum ssp. plicatum capitulums in streptozotocin-induced-diabetic rats. J Ethnopharmacol, 109:54-59. |
| [8] | Atawodi, S.E., Atawodi, J.C., Idakwo, G. A., Pfundstein, B., Haubner, R., Wurtele, G., Bartsch,H., and Owen, R. W. (2010). Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem, and root barks of Moringa oleifera Lam. J. Med. Food 13, 710–716. |
| [9] | Ayala, J. E., Bracy, D. P., McGuinness, O. P. and Wasserman, D. H. (2006). Considerations in the design of hyperinsulinemic-euglycemic clamps in the conscious mouse. Diabetes 55, 390-397. |
| [10] | Beach E.F, Turner J.J. (1958). An enzymatic method for glucose determination in body fluids. Clin. Chem., 4:462-475. |
| [11] | Bennett RN, FA Mellon, N Foidl, JH Pratt, MS DuPont, L Perkins and PA Kroon (2003) Profiling glucosinolates and phenolics in vegetative and reproductive tissues of the multi-purpose trees Moringa oleifera L. (Horseradish tree) and Moringa stenopetala L. Journal of Agricultural and Food Chemistry 51: 3546-3553. |
| [12] | Bortolotti, M., Levorato, M., Lugli, A., and Mazzero, G. (2008). Effect of a balanced mixture of dietary fibers on gastric emptying, intestinal transit and body weight. Ann .Nutr. Metab. 52, 221–226. |
| [13] | Chandrasekhar. (2012). Evaluation of hypoglycemic and antihyperglycemic effect of freshly prepared aqueous extract of Moringa oleifera leaves in normal and diabetic rabbits J. Chem. Pharm. Res., , 4(1):249-253. |
| [14] | Cheenpracha, S., Park, E.J., Yoshida, W.Y., Barit, C., Wall, M., Pezzuto, J. M., and Chang, L.C.(2010). Potential anti-inflammatory phenolic glycosides from the medicinal plant Moringa oleifera fruits. Bioorg. Med. Chem. 18, 6598–6602. |
| [15] | Chumark, P., Khunawat, P., Sanvarinda, Y., Phornchirasilp, S., Morales, N.P., Phivthong-Ngam, L., Ratanacham-nong, P., Srisawat, S., and Pon-grapeeporn, K.U. (2008).The in vitro and ex vivo antioxidant properties, hypolipidaemic and anti atherosclerotic activities of water extract of Moringa oleifera Lam. leaves. J. Ethnopharmacol. 116, 439–446. |
| [16] | Coppin,J. (2008). A Study of the Nutritional and Medicinal Values of Moringa oleifera Leaves from sub-Saharan Africa: Ghana, Rwanda, Senegal and Zambia. M.Sc.thesis, Rutgers University-Graduate School, New Brunswick, NewBrunswick |
| [17] | De Fronzo RA, Ferrannini E, Simonson DC. (1989). Fasting hyperglycemia in non-insulin dependent diabetes mellitus: contributions of excessive hepatic glucose production and impaired tissue glucose uptake. Metabolism; 38:387-395 |
| [18] | De Fronzo RA. (1999). Pharmacologic therapy for Type diabetes mellitus. Ann Intern Med; 131:281-303 |
| [19] | De Fronzo RA.(1997). Pathogenesis of Type 2 diabetes: metabolic and molecular implication for identifying diabetes genes. Diabetes Res. 5:177-269 |
| [20] | DeFronzoRA, Ferrannini E. (1991). Insulin resistance. A multifaceted syndrome responsible for NIDDM, obesity, hypertension, dyslipidemia, and atherosclerotic cardiovascular disease. Diabetes Care 14: 173–194. |
| [21] | Dhalla, N.S., Temsah, R.M., and Netticadan, T. (2000). Role of oxidative stress in cardiovascular diseases. J. Hypertens. 18, 655–673. |
| [22] | Dolly Jaiswal, Prashant Kumar Rai, Amit Kumar, Shikha Mehta, GeetaWatal. (2009). Journal of Ethnopharmacology;123:392–396. |
| [23] | Fahey JW, AT Zalcmann, and P Talalay (2001). The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56(1): 5-51. [corrigendum: Phytochemistry 59: 237]. |
| [24] | Ferarannini E, Simonson DC, Katz LD, Reichard G, Bevilacqua S, Barrett EJ, et al. (1988). The disposal of an oral glucose load in patients with non-insulin dependent diabetes. Metabolism; 37:79-85 |
| [25] | Finch CF, Zimmet PZ, Alberti KGMM. (1990). Determining diabetes prevalence: a rational basis for the use of fasting plasma glucose concentration. Diabetes Medicine; 7:603-10. |
| [26] | Food and Agriculture Organization of the United Nations. (1999). Country Pasture/Forage Resource Profiles: Philippines |
| [27] | Fuglie LJ (1999) The Miracle Tree: Moringa oleifera: Natural Nutrition for the Tropics. Church World Service, Dakar. 68 pp.; revised in 2001 and published as The Miracle Tree: The Multiple Attributes of Moringa, 172 pp. |
| [28] | Galigher AE and Kozloff EN (1971). Essentials Practical Microtechnique. Lea and Feigner, Philadelphia 2nd edition, pg.77-82. |
| [29] | Gandhipuram, P.S.K., A. Palanisamy, S.K. Durairaj and P.S. Sorimuthu. (2006). Anti diabetic activity of fruits of Terminalia chebula on streptozotocin-induced diabetic rats. J. Health Sci., 52: 283-291. |
| [30] | Ghasi S, Nwobodo E, Ofili J.O. (2007). Hypercholesterolemic effects of crude extract of leaf of Moringa Oleifera Lam in high fat diet fed Wistar rats. Journal of Ethnopharmacology , 21-25. |
| [31] | Ghiridhari, V.V.A., Malhati, D., and Geetha, K. (2011). Anti-diabetic properties of drumstick (Moringa oleifera) leaf tablets. Int.J.Health Nutr. 2, 1–5. |
| [32] | Gupta,A., Gautam, M.K., Singh, R. K., Kumar, M.V., RaoCh, V., Goel, R.K., and Anupurba, S. (2010). Immunomodulatory effect of Moringa oleifera Lam. extracton cyclophosphamide induced toxicity in mice. Indian J Exp. Biol. 48, 1157–1160. |
| [33] | Han, B. G., Hao, C. M., Tchekneva, E. E., Wang, Y. Y., Lee, C. A., Ebrahim, B., Harris, R. C., Kern, T. S., Wasserman, D. H., Breyer, M. D. et al. (2008). Markers of glycemic control in the mouse: comparisons of 6-hand overnight-fasted blood glucoses to Hb A1c. Am. J. Physiol. Endocrinol. Metab. 295, E981-E986. |
| [34] | Hanley AJG, Stern MP, Williams K, Haffner SM. (2002). Homeostasis model assessment of insulin resistance in relation to the incidence of cardiovascular disease. Diabetes Care, 25:1177-1184. |
| [35] | Heijboer, A. C., Donga, E., Voshol, P. J., Dang, Z. C., Havekes, L. M., Romijn, J. A. and Corssmit, E. P. (2005). Sixteen hours of fasting differentially affects hepatic and muscle insulin sensitivity in mice. J. Lipid Res. 46, 582-588. |
| [36] | HimsworthHP. (1936). Diabetes mellitus: its differentiation into insulin-sensitive and insulin-insensitive types. Lancet 227: 127–130. |
| [37] | http://www.echotech.org/bookstore/advanced_search_result.php?keywords=Miracle+Tree. |
| [38] | International Diabetes Federation, IDF, Diabetes Atlas. Global Burden. Epidemiology and Morbidity. Diabetes and Impaired Glucose Tolerance. Available at http://www.diabetesatlas.org/content/diabetes-and-impaired-glucose-tolerance (assessed 19th July 2011). |
| [39] | Islam MS, Choi H, Loots du T (2008). Effects of dietary onion (Allium Cepa L.) in a high-fat diet streptozotocin-induced diabetes rodent model. Ann Nutr Metab, 53:6-12. |
| [40] | Jain, P.J., Patil, S.D., Haswani, N. G., Girase, M.V., and Surana, S. J. (2010). Hypolipidemic activity of Moringa oleifera Lam., Moringaceae, on high fat diet-induced hyperlipidemia in albin o rats. Braz.J.Pharmacogn. 20, 969–973. |
| [41] | Ji Young Jung, Yeni Lim, Min Sun Moon, Ji Yeon Kim and Oran Kwon. (2011) Onion peel extracts ameliorate hyperglycemia and insulin resistance in high fat diet/streptozotocin-induced diabetic rats. Nutrition & Metabolism, 8:18 |
| [42] | Joshi, P., and Mehta, D.(2010). Effect of dehydration on the nutritive value of drumstick leaves. Metabolomics 1, 5–9. |
| [43] | Kar A, Choudhary BK and Bandyopadhyay NG. (2003). J Ethnopharmacol. 84(1):105-108. |
| [44] | Limon-Pacheco, J., and Gonsebatt, M.E. (2009). The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutat. Res. 674, 137–147. |
| [45] | Lin, X., Racette, S.B., Lefevre, M., Spearie, C.A., Most, M., Ma, L., and Ostlund, R.E. Jr. (2010). The effects of phytosterols present in natural food matrices on cholesterol metabolism and LDL-cholesterol: a controlled feeding trial. Eur.J.Clin. Nutr. 64, 1481–1487. |
| [46] | Mahajan, S., and Mehta, A. (2009). Curative effect of hydroalcoholic extract of leaves of Moringa oleifera lam. Against adjuvant induced established arthritis in rats. Niger. J. Nat. Prod. Med. 13, 13–22. |
| [47] | Mahajan, S.G., and Mehta, A.A. (2010). Immunosuppressive activity of ethanolic extract of seeds of Moringa oleifera Lam. In exper imental immune inflammation. J. Ethnopharmacol. 130, 183–186. |
| [48] | Manohar. V. S, T. Jayasree, K. Kiran Kishore, L. Mohana Rupa, Rohit Dixit and N. |
| [49] | Marles, R.J. and N.R. Farnsworth. (1995). Phytomedicine., 2:137-187. |
| [50] | Mathew DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. (1985) Homeostasis model assessment: Insulin resistance and beta-cell function from fasting glucose and insulin concentrations in man. Diabetologia; 28:412-419. |
| [51] | Matsui T, Ogunwande LA, Abesundara KJM, Matsumoto K (2006). Antihyperglycemic potential of natural products. Mini-Rev Med Chem, 6:109-120. |
| [52] | Maxwell C.S, Wood R.J. (2011). Update on vitamin D and Type 2 diabetes. Nutrition Reviews; 69(5):291-295 |
| [53] | Mehta, K., Balaraman, R., Amin, A. H., Bafna, P.A., and Gulati, O.D. (2003). Effect of fruits of Moringa oleifera on the lipid profile of normal and hypercholesterolaemic rabbits. J. Ethnopharmacol. 86, 191–195. |
| [54] | Miller LC, Tainter ML (1944). Estimation of LD50 and its error by means of log-probit graph paper. Proc Soc Exp Bio Med; 57:261. |
| [55] | Muangnoi, C., Chingsuwanrote, P., Praengamthanachoti, P., Svasti, S., andTuntipopipat, S. (2011). Moringa oleifera pod inhibits inflammatory mediator production by lipopolysaccharide-stimulated RAW264.7 murine macrophage cell lines. Inflammation. doi: 10.1007/s10753-011-9334-4. [Epub ahead ofprint]. |
| [56] | Naznin Ara, Mamunur Rashid and Md. Shah Amran. (2008). Saudi Journal of Biological Sciences;15 (2):253-258. |
| [57] | Ndong, M., Uehara, M., Katsumata, S., and Suzuki, K. (2007b). Effects of oral administration of Moringa oleifera Lam on glucose tolerance in Goto-Kakizaki and Wistarrats. J. Clin. Biochem. Nutr. 40, 229–233. |
| [58] | O’Sullivan JB, Mahan CM. (1964). Criteria for the oral glucose tolerance test in pregnancy. Diabetes 13: 278 |
| [59] | Okigbo, R.N. and E.C. Mmeka. (2006). An appraisal of phytomedicine in Africa. KMITL Sci. Tech. J., 6: 83-94. |
| [60] | Panda S, Kar A (2007). Antidiabetic and antioxidative effects of Annona squamosa leaves are possibly mediated through quercetin-3-O-glucoside. Biofactors, 31:201-210. |
| [61] | Parveen K, Khan MR, Mujeeb M, Siddiqui WA (2010). Protective effects of Pycnogenol on hyperglycemia-induced oxidative damage in liver of type 2 diabetic rats. Chem Biol Interact, 186:219-227. |
| [62] | Petersen KF, Dufour S, Savage DB, Bilz S, Solomon G, Yonemitsu S, Cline GW, Befroy D, Zemany L, Kahn BB, Papademetris X, Rothman DL, Shulman GI. (2004). The role of skeletal muscle insulin resistance in the pathogenesis of the metabolic syndrome. Proc Natl Acad Sci USA 104: 12587–12594. |
| [63] | Pharmacol Rev, 22:485-518. |
| [64] | Piedrola, G., E. Novo, F. Escober and R. Garcia-Robles, (2001). White blood cell count and insulin resistance in patients with coronary artery disease. Ann. Endocrinol., (Paris), 62: 7-10. |
| [65] | Rai. P.K., Jaiswal. D., Diwakar. S., Watal. G. (2008). Pharmaceutical Biology,46:360–365. |
| [66] | Rai. P.K., Rai. N.K., Rai. A.K., Watal. G. (2007). Instrumentation Science and Technology. 35, 507–522. |
| [67] | Rajangam J., et al. ( 2001). "Status of Production and Utilisation of Moringa in Southern India". Development potential for Moringa products (Dar es Salaam, Tanzania). http://www.moringanews.org/actes/rajangam_en.doc. |
| [68] | Rana, J.S., Nieuwdorp, M., Jukema, J. W., and Kastelein, J.J. (2007). Cardiovascular metabolic syndrome – an interplay of obesity, inflammation, diabetes and coronary heart disease. Diabete sObes. Metab. 9, 218–232. |
| [69] | ReavenGM. (2005). The insulin resistance syndrome: definition and dietary approaches to treatment. Annu Rev Nutr 25: 391–406 |
| [70] | Reed MJ, Meszaros K, Entes LJ, Claypool MD, Pinkett JG, Gadbois TM, Reaven GM (2000). A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat. Metabolism, 49:1390-1394. |
| [71] | Rerup CC (1970). Drugs producing diabetes through damage of the insulin secreting cells. |
| [72] | Rheney C.C, Kirk K.K. (2000). Performance of three blood glucose meters. Ann. Pharmacother., March, 34(3):317-321. |
| [73] | Ryter, S.W., Kim, H.P., Hoetzel, A., Park, J.W., Nakahira, K., Wang, X., and Choi, A.M. (2007). Mechanisms of cell death in oxidative stress. Antioxid. Redox Signal. 9, 49–89. |
| [74] | Santosh Kumar Singh, Prashant Kumar Rai, Dolly Jaiswal, and Geeta Watal. (2008). Evid Based Complement Alternat Med., 5(4):415–420. |
| [75] | Schteingart D.E. (1997). Glucose metabolism and Diabetes mellitus. In: Price S.E, Nilson L.E eds. Pathophysiology; Clinical concepts of disease processes, 5th ed. Missouri; Mosby;954-695 |
| [76] | Sharma, S.R., Dwivedi, S.K., Swarup, D. (2008). Indian Journal of Experimental Biology. 34:372–374. |
| [77] | Shaw J, Sicree R. (2009). Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Researcch and clinical Practice; 87:4-14 |
| [78] | Singh, S.K. Kesari, A.N. Gupta, R.K., Jaiswal, D. Watal, G. (2007). Journal of Ethnopharmacology, 114:174–179. |
| [79] | Sofowora A. (1993). Medicinal plants and Traditional Medicine in African. Spectrum Books Ltd., Ibadan, Nigeria, pp:150-153. |
| [80] | Sreelatha, S. ,and Padma, P.R. (2009). Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity. Plant Foods Hum. Nutr. 64, 303–311. |
| [81] | Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P (2005). Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening. Pharmacol Res, 52:313-320. |
| [82] | Stumvoll M, Goldstein BJ, van Haeften TW (2005). Type 2 diabetes: principles of pathogenesis and therapy. Lancet, 365:1333-1346. |
| [83] | Sudha, P., Asdaq, S.M., Dhamingi, S. S., and Chandrakala, G.K. (2010). Immuno modulatory activity of methanolic leaf extract of Moringa oleifera in animals. Indian J. Physiol .Pharmacol. 54, 133–140. |
| [84] | Sulaiman, M.R., Zakaria, Z.A., Bujarimin, A.S., Somchit, M.N., Israf, D.A., and Moin, S. (2008). Evaluation of Moringa oleifera aqueous extract for anti nociceptive and anti-inflammatory activities in animal models. Pharmacol. Biol. 46, 838–845. |
| [85] | Teixeira, C.C., Rava C.A., Da Silva P.M., Melchior R, Argenta R, Anselmi F, Almeida A.F.C., Fuchs F.D. (2000). Absence of and hyperglycemic effect of jambolan in experimental and clinical models. J. Ethnopharmacol., 71: 343-347. |
| [86] | Tende J.A, Ezekiel L, Dikko A.A.U, Goji A.D.T. (2011). Effect of Ethanolic leaves extract of Moringa oleifera on blood glucose levels of streptozocin-induced diabetics and normoglycemic Wistar rats. Br. J. Pharm. Toxicol., 2(1): 1-4. |
| [87] | Trease G.E, Evans M.S. (1989). Textbook of Pharmacognosy. 14th Edn., Balliere Tindall, London, pp: 81-90,269-275,300. |
| [88] | Verma, A.R., Vijayakumar, M., Mathela, C. S., and Rao, C.V. (2009). Invitro and invivo antioxidant properties of different fractions of Moringa oleifera leaves. Food Chem. Toxicol. 47, 2196–2201. |
| [89] | Wild, S., G. Roglic, A. Green, R. Sicree and H. King. (2004). Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care, 27: 1047-1053 |
| [90] | Wolever TM, Jenkins DJ, Jenkins AL, Josse RG. (1991): The glycemic index: methodology and clinical implications. Am J Clin Nutr, 54:846-854. |
| [91] | World Health Organization (1999). Definition, Diagnosis and classification of Diabetes mellitus and its complications. Report of WHO part 1 Geneva (WHO / NCD/NCS/1992). |
| [92] | Yaryura-Tobias, J.A., A. Pinto and F. Neziroglu. (2001). Anorexia nervosa, diabetes mellitus, brain atrophy and fatty liver. Int. J. Eating Disorders, 30: 350-353. |
APA Style
Anyanwu Anthony Chinedu., Salako Olanrewaju Alani., Adeyemi Olufunmi Olaide. (2015). Effect of the Ethanolic Leaf Extract of Moringa oleifera on Insulin Resistance in Streptozotocin Induced Diabetic Rats. Journal of Plant Sciences, 2(6-1), 5-12. https://doi.org/10.11648/j.jps.s.2014020601.12
ACS Style
Anyanwu Anthony Chinedu.; Salako Olanrewaju Alani.; Adeyemi Olufunmi Olaide. Effect of the Ethanolic Leaf Extract of Moringa oleifera on Insulin Resistance in Streptozotocin Induced Diabetic Rats. J. Plant Sci. 2015, 2(6-1), 5-12. doi: 10.11648/j.jps.s.2014020601.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.jps.s.2014020601.12,
author = {Anyanwu Anthony Chinedu. and Salako Olanrewaju Alani. and Adeyemi Olufunmi Olaide.},
title = {Effect of the Ethanolic Leaf Extract of Moringa oleifera on Insulin Resistance in Streptozotocin Induced Diabetic Rats},
journal = {Journal of Plant Sciences},
volume = {2},
number = {6-1},
pages = {5-12},
doi = {10.11648/j.jps.s.2014020601.12},
url = {https://doi.org/10.11648/j.jps.s.2014020601.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.s.2014020601.12},
abstract = {Insulin resistance plays a central role in the pathogenesis of type 2 diabetes mellitus. Ameliorating insulin resistance helps to improve glycaemic control and hence reduce the risk of development and progression of diabetic complications. The extract of Moringa oleifera has been shown to improve hyperglycaemia, however, little is known on its effect on insulin resistance and beta-cell function. The objective of this study was to determine the effect of the ethanolic leaf extract of Moringa oleifera on insulin resistance in high-fat diet and streptozotocin induced diabetic rats. Different doses (250 and 500 mg/kg) of the ethanolic leaf extract of Moringa oleifera were administered orally for two weeks, to high-fat diet and streptozotocin induced diabetic rats, and were compared with metformin 320 mg/kg and control group (distilled water 10 ml/kg) for the effect on fasting blood glucose, serum insulin and insulin resistance (HOMA). The extract of Moringa oleifera (at doses of 250 mg/kg and 500 mg/kg) significantly lowered the fasting blood glucose at days 7 and 14 compared to controls (p = 0.0021 and p = 0.0001 respectively). There was a significant increase in serum insulin level in the control group at days 7 and 14 compared to the groups treated with the extract (250 mg/kg, 500 mg/kg) and metformin (p<0.01, p<0.02 and p<0.01 respectively). The extract at both doses and metformin (320 mg/kg) induced a significant improvement in insulin resistance (HOMA-IR) on days 7 and 14 (p < 0.0001).},
year = {2015}
}
TY - JOUR T1 - Effect of the Ethanolic Leaf Extract of Moringa oleifera on Insulin Resistance in Streptozotocin Induced Diabetic Rats AU - Anyanwu Anthony Chinedu. AU - Salako Olanrewaju Alani. AU - Adeyemi Olufunmi Olaide. Y1 - 2015/03/14 PY - 2015 N1 - https://doi.org/10.11648/j.jps.s.2014020601.12 DO - 10.11648/j.jps.s.2014020601.12 T2 - Journal of Plant Sciences JF - Journal of Plant Sciences JO - Journal of Plant Sciences SP - 5 EP - 12 PB - Science Publishing Group SN - 2331-0731 UR - https://doi.org/10.11648/j.jps.s.2014020601.12 AB - Insulin resistance plays a central role in the pathogenesis of type 2 diabetes mellitus. Ameliorating insulin resistance helps to improve glycaemic control and hence reduce the risk of development and progression of diabetic complications. The extract of Moringa oleifera has been shown to improve hyperglycaemia, however, little is known on its effect on insulin resistance and beta-cell function. The objective of this study was to determine the effect of the ethanolic leaf extract of Moringa oleifera on insulin resistance in high-fat diet and streptozotocin induced diabetic rats. Different doses (250 and 500 mg/kg) of the ethanolic leaf extract of Moringa oleifera were administered orally for two weeks, to high-fat diet and streptozotocin induced diabetic rats, and were compared with metformin 320 mg/kg and control group (distilled water 10 ml/kg) for the effect on fasting blood glucose, serum insulin and insulin resistance (HOMA). The extract of Moringa oleifera (at doses of 250 mg/kg and 500 mg/kg) significantly lowered the fasting blood glucose at days 7 and 14 compared to controls (p = 0.0021 and p = 0.0001 respectively). There was a significant increase in serum insulin level in the control group at days 7 and 14 compared to the groups treated with the extract (250 mg/kg, 500 mg/kg) and metformin (p<0.01, p<0.02 and p<0.01 respectively). The extract at both doses and metformin (320 mg/kg) induced a significant improvement in insulin resistance (HOMA-IR) on days 7 and 14 (p < 0.0001). VL - 2 IS - 6-1 ER -
Email: