<i>Ex Situ</i> Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent

Amechi Sampson Nwankwegu; Emmanuel Michael Ikpe; Edna Ifeoma Chukwura; Richard Chidiebere Irondi; Emmanuel Ifeanyi Obika

doi:doi:10.11648/j.ajls.20160403.11

Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent

Amechi Sampson Nwankwegu, Emmanuel Michael Ikpe, Edna Ifeoma Chukwura, Richard Chidiebere Irondi, Emmanuel Ifeanyi Obika

Published in American Journal of Life Sciences (Volume 4, Issue 3)

Received: 18 May 2016 Accepted: 27 May 2016 Published: 14 June 2016

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard methods, which includes screening, inoculums development and degradation studies to assess the most promising hydrocarbon degraders. Pooled samples collected from different points on the palm oil mill effluent disposal channels were inoculated mineral salt medium containing crude oil to assess degradation abilities of different bacterial species. Data indicated that these bacterial isolates were capable of thriving in the mineral salt medium between the pH range of 6.0-7.0 and bacterial proliferation were quantified by assessing the total viable count which ranged between 2 x 10⁵-9 x 10⁵ cfu/ml based on individual abilities to utilize crude oil as a sole carbon source. Cultural, morphological and biochemical characterizations were conducted on these isolates and Bergey’s manual of determinative bacteriology was used to suggest possible isolates, while molecular identification was done Centre for Agriculture and biosciences International (CABI), UK using partial 16S rDNA sequencing analysis and FASTA algorithm with prokaryote database from EBI. Escherichia fergosonii, Klebsiella variicola and Microccocus luteus were identified. Gas chromatographic analysis was applied to quantify the extent of degradation of crude oil by the isolates. All the isolated bacterial species displayed varying degree of crude hydrocarbon biodegradation.

Published in	American Journal of Life Sciences (Volume 4, Issue 3)
DOI	10.11648/j.ajls.20160403.11
Page(s)	71-75
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Palm Oil Mill Effluent, Crude Oil, Biodegradation, Bacteria, Ex Situ

References

[1]	Kvenvolden K. A. Cooper C. K.. Natural seepage of crude oil into the marine environment”, Geo- marine letters. Vol. 23, 2003, pp. 140-146.
[2]	Tanaka, O. H., Venkatswaran, K., Komukai, S., Toki, H., Iwabuchi, T. And Miyachi, S. Ecodynamics of oil degrading bacterial and significance of marine mixed population in the degradation of petroleum compounds. International oil spill conference. 1993, pp. 780-785.
[3]	Yoshida, M. H. Michel, L. A. Sazanov, S. Yoshikawa, J. Barber, J. K. Lanyi, S. P. Balashov, V. A. Shuvalov, A. G. Yakovlev, T. A. Shkuropatova, L. G. Vasiheva, A. Y. Shkuropatov, P. Gast, S. D. Dunn, P. A. Delrizzo, Y. Bi, K. S. Wood, D. J. Cipriano, P. Turina, A. Biochimica et Biophsica Acta (BBA)-Bioenergetics, 2006, Vol. 1757, 151-155.
[4]	Odu, C. I. I. Microbiology of soil contaminated with petroleum hydrocarbons. The extent of contamination of some soils and microbial properties after contamination. Journal of Institute of Petroleum. 1972, vol 58, pp. 202-208.
[5]	Jameel, A. T, Olanrewwaju, A. A. Aerobic biodegradation of oil and grease in palm oil mill effluent using consortium of microorganisms. In: M. D. Z, Alam, A. T, Jameel and A, Amid (eds). Current research and development in biotechnology engineering at International Islamic University Malaysia (IIUM) Vol. III. IIUM Press, Kula Lumpur. 2011, pp. 43-51. ISBN 978967418444
[6]	Nwankwegu, A. S., Onwosi, C. O., Orji, M. U., Anaukwu, C. G., Okafor, U. C., Azi, F., Marins, P. E. Reclamation of DPK hydrocarbon polluted agricultural soil using a selected bulking agent. Journal of Environmental Management. 2016, Vol. 172, pp. 136-142.
[7]	Obayori, O. S., Ilori, M. O., O. S., Ilori., Adebusoye, M. O., Amund, O. O. and Oyetibe. Microbial population changes in tropical agricultural soil experimentally contaminated with crude oil petroleum. African Journal of Biotechnology. 2008, pp. 4512-4520.
[8]	Okpokwasili, G. C and Okori, B. B. Biodeterioration potentials of Microorganisms isolated from car engine lubricating oil. Tribology International. 1988, Vol. 21, pp. 215-220.
[9]	Obuekwe, C. O and Al-zarban. Bioremediation of crude oil polluted in Kuwaiti desert: The role of adherent microorganisms, Environmental International. 2011, Vol. 24, pp. 832-834.
[10]	Bartha, R and Atlas, R. M. The Microbiology of Aquatic oil spills. Advance Applied Microbiology Vol. 22, pp. 225-226.
[11]	Calomiris, J. T., Austin, B, Walker, J. D. and Colwell, R. R. Enrichment for Estuarine. Petroleum Degrading Bacterial using liquid and solid media. Journal of Applied Bacteriology. 1976. Vol. 41, pp. 135-144.
[12]	Pacwa-Plociniczak, M., Plaza, G. A., Pitrowska-seget, Z. M. Monitoring the changes in a bacterial community in petroleum-polluted soil bioaugmented with hydrocarbon-degrading strains. Applied Soil Ecology. 2016, Vol. 105, pp. 76-85.
[13]	Stordal, I. F., Olsen, A. J., Jenssen, B. M., Netzer, R., Hansen, B. H., Altin, D., Braskstad, O. G. Concentrations of viable oil-degrading microorganisms are increased in feces from Calanus finmarchicus feeding in petroleum oil dispersions. Marine pollution Bulletin. 2015, Vol. 98, pp. 69-77.
[14]	Ntougias, S., Melidis, P., Navrozidou, E., Tzegkas, F. Diversity and efficiency of anthracene-degrading bacteria isolated from a denitrifying activated sludge system treating municipal wastewater. International Biodeterioration & Biodegradation. 2015, Vol. 97, pp. 151-158.
[15]	Kurachi, K., Hosokawa, R., Takahashi, M., Okuyama, H. The potential of glycerol in freezing preservation of turbine oil-degrading bacterial consortium and the ability of the revised consortium to degrade petroleum wastes. International Biodeterioration & Biodegradation. 2014, Vol. 88, pp. 77-82.

Cite This Article

Plain Text BibTeX RIS

APA Style

Amechi Sampson Nwankwegu, Emmanuel Michael Ikpe, Edna Ifeoma Chukwura, Richard Chidiebere Irondi, Emmanuel Ifeanyi Obika. (2016). Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent. American Journal of Life Sciences, 4(3), 71-75. https://doi.org/10.11648/j.ajls.20160403.11

Copy | Download

ACS Style

Amechi Sampson Nwankwegu; Emmanuel Michael Ikpe; Edna Ifeoma Chukwura; Richard Chidiebere Irondi; Emmanuel Ifeanyi Obika. Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent. Am. J. Life Sci. 2016, 4(3), 71-75. doi: 10.11648/j.ajls.20160403.11

Copy | Download

AMA Style

Amechi Sampson Nwankwegu, Emmanuel Michael Ikpe, Edna Ifeoma Chukwura, Richard Chidiebere Irondi, Emmanuel Ifeanyi Obika. Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent. Am J Life Sci. 2016;4(3):71-75. doi: 10.11648/j.ajls.20160403.11

Copy | Download

@article{10.11648/j.ajls.20160403.11,
  author = {Amechi Sampson Nwankwegu and Emmanuel Michael Ikpe and Edna Ifeoma Chukwura and Richard Chidiebere Irondi and Emmanuel Ifeanyi Obika},
  title = {Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {3},
  pages = {71-75},
  doi = {10.11648/j.ajls.20160403.11},
  url = {https://doi.org/10.11648/j.ajls.20160403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160403.11},
  abstract = {Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard methods, which includes screening, inoculums development and degradation studies to assess the most promising hydrocarbon degraders. Pooled samples collected from different points on the palm oil mill effluent disposal channels were inoculated mineral salt medium containing crude oil to assess degradation abilities of different bacterial species. Data indicated that these bacterial isolates were capable of thriving in the mineral salt medium between the pH range of 6.0-7.0 and bacterial proliferation were quantified by assessing the total viable count which ranged between 2 x 105-9 x 105 cfu/ml based on individual abilities to utilize crude oil as a sole carbon source. Cultural, morphological and biochemical characterizations were conducted on these isolates and Bergey’s manual of determinative bacteriology was used to suggest possible isolates, while molecular identification was done Centre for Agriculture and biosciences International (CABI), UK using partial 16S rDNA sequencing analysis and FASTA algorithm with prokaryote database from EBI. Escherichia fergosonii, Klebsiella variicola and Microccocus luteus were identified. Gas chromatographic analysis was applied to quantify the extent of degradation of crude oil by the isolates. All the isolated bacterial species displayed varying degree of crude hydrocarbon biodegradation.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent
AU  - Amechi Sampson Nwankwegu
AU  - Emmanuel Michael Ikpe
AU  - Edna Ifeoma Chukwura
AU  - Richard Chidiebere Irondi
AU  - Emmanuel Ifeanyi Obika
Y1  - 2016/06/14
PY  - 2016
N1  - https://doi.org/10.11648/j.ajls.20160403.11
DO  - 10.11648/j.ajls.20160403.11
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 71
EP  - 75
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20160403.11
AB  - Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard methods, which includes screening, inoculums development and degradation studies to assess the most promising hydrocarbon degraders. Pooled samples collected from different points on the palm oil mill effluent disposal channels were inoculated mineral salt medium containing crude oil to assess degradation abilities of different bacterial species. Data indicated that these bacterial isolates were capable of thriving in the mineral salt medium between the pH range of 6.0-7.0 and bacterial proliferation were quantified by assessing the total viable count which ranged between 2 x 105-9 x 105 cfu/ml based on individual abilities to utilize crude oil as a sole carbon source. Cultural, morphological and biochemical characterizations were conducted on these isolates and Bergey’s manual of determinative bacteriology was used to suggest possible isolates, while molecular identification was done Centre for Agriculture and biosciences International (CABI), UK using partial 16S rDNA sequencing analysis and FASTA algorithm with prokaryote database from EBI. Escherichia fergosonii, Klebsiella variicola and Microccocus luteus were identified. Gas chromatographic analysis was applied to quantify the extent of degradation of crude oil by the isolates. All the isolated bacterial species displayed varying degree of crude hydrocarbon biodegradation.
VL  - 4
IS  - 3
ER  -

Copy | Download

Author Information

Amechi Sampson Nwankwegu

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
Emmanuel Michael Ikpe

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
Edna Ifeoma Chukwura

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
Richard Chidiebere Irondi

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
Emmanuel Ifeanyi Obika

Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Amechi Sampson Nwankwegu, Emmanuel Michael Ikpe, Edna Ifeoma Chukwura, Richard Chidiebere Irondi, Emmanuel Ifeanyi Obika. (2016). Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent. American Journal of Life Sciences, 4(3), 71-75. https://doi.org/10.11648/j.ajls.20160403.11

Copy | Download

ACS Style

Amechi Sampson Nwankwegu; Emmanuel Michael Ikpe; Edna Ifeoma Chukwura; Richard Chidiebere Irondi; Emmanuel Ifeanyi Obika. Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent. Am. J. Life Sci. 2016, 4(3), 71-75. doi: 10.11648/j.ajls.20160403.11

Copy | Download

AMA Style

Amechi Sampson Nwankwegu, Emmanuel Michael Ikpe, Edna Ifeoma Chukwura, Richard Chidiebere Irondi, Emmanuel Ifeanyi Obika. Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent. Am J Life Sci. 2016;4(3):71-75. doi: 10.11648/j.ajls.20160403.11

Copy | Download

@article{10.11648/j.ajls.20160403.11,
  author = {Amechi Sampson Nwankwegu and Emmanuel Michael Ikpe and Edna Ifeoma Chukwura and Richard Chidiebere Irondi and Emmanuel Ifeanyi Obika},
  title = {Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent},
  journal = {American Journal of Life Sciences},
  volume = {4},
  number = {3},
  pages = {71-75},
  doi = {10.11648/j.ajls.20160403.11},
  url = {https://doi.org/10.11648/j.ajls.20160403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20160403.11},
  abstract = {Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard methods, which includes screening, inoculums development and degradation studies to assess the most promising hydrocarbon degraders. Pooled samples collected from different points on the palm oil mill effluent disposal channels were inoculated mineral salt medium containing crude oil to assess degradation abilities of different bacterial species. Data indicated that these bacterial isolates were capable of thriving in the mineral salt medium between the pH range of 6.0-7.0 and bacterial proliferation were quantified by assessing the total viable count which ranged between 2 x 105-9 x 105 cfu/ml based on individual abilities to utilize crude oil as a sole carbon source. Cultural, morphological and biochemical characterizations were conducted on these isolates and Bergey’s manual of determinative bacteriology was used to suggest possible isolates, while molecular identification was done Centre for Agriculture and biosciences International (CABI), UK using partial 16S rDNA sequencing analysis and FASTA algorithm with prokaryote database from EBI. Escherichia fergosonii, Klebsiella variicola and Microccocus luteus were identified. Gas chromatographic analysis was applied to quantify the extent of degradation of crude oil by the isolates. All the isolated bacterial species displayed varying degree of crude hydrocarbon biodegradation.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Ex Situ Biodegradation of Crude Oil Using Bacterial Isolates From Palm Oil Mill Effluent
AU  - Amechi Sampson Nwankwegu
AU  - Emmanuel Michael Ikpe
AU  - Edna Ifeoma Chukwura
AU  - Richard Chidiebere Irondi
AU  - Emmanuel Ifeanyi Obika
Y1  - 2016/06/14
PY  - 2016
N1  - https://doi.org/10.11648/j.ajls.20160403.11
DO  - 10.11648/j.ajls.20160403.11
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 71
EP  - 75
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.20160403.11
AB  - Bioremediation of crude oil is quite unique, complex and relatively a slow process. They study area for this research work was a cottage industry located in Orumba North local government area of Anambra state, Nigeria. Evaluating the degradation potential of bacterial isolates from palm oil mill effluent on crude oil was conducted using standard methods, which includes screening, inoculums development and degradation studies to assess the most promising hydrocarbon degraders. Pooled samples collected from different points on the palm oil mill effluent disposal channels were inoculated mineral salt medium containing crude oil to assess degradation abilities of different bacterial species. Data indicated that these bacterial isolates were capable of thriving in the mineral salt medium between the pH range of 6.0-7.0 and bacterial proliferation were quantified by assessing the total viable count which ranged between 2 x 105-9 x 105 cfu/ml based on individual abilities to utilize crude oil as a sole carbon source. Cultural, morphological and biochemical characterizations were conducted on these isolates and Bergey’s manual of determinative bacteriology was used to suggest possible isolates, while molecular identification was done Centre for Agriculture and biosciences International (CABI), UK using partial 16S rDNA sequencing analysis and FASTA algorithm with prokaryote database from EBI. Escherichia fergosonii, Klebsiella variicola and Microccocus luteus were identified. Gas chromatographic analysis was applied to quantify the extent of degradation of crude oil by the isolates. All the isolated bacterial species displayed varying degree of crude hydrocarbon biodegradation.
VL  - 4
IS  - 3
ER  -

Copy | Download