The objective of this case control study is to identify the relationship between intestinal parasitic infection and Irritable Bowel Syndrome (IBS) among Sudanese patients. Method: Two hundred patients diagnosed with irritable bowel syndrome (IBS) and were attended Health Facilities at Khartoum State were enrolled, together with 99 apparently healthy controls. Patients were classified into three groups (IBS-all types, IBS with diarrhea (IBS-D), and IBS with constipation (IBS-C)). Stool specimens were collected form study population and all were subjected to microscopic examination following wet saline preparation, formal-ether concentration technique, Z. N stain, and in vitro cultivation in Locke egg medium. Results: Intestinal parasites were detected in 32% (64/200) of IBS-all types; and in 16.2% (16/99) of control group (P. value 0.005). For patients, 20 (10%) were positive for the Entamoeba cyst, 3 (1.5%) Giardia lamblia cysts, and 41 (20.5%) were positive for Blastocystis hominis cyst. For control, Entamoeba species were detected in 10 (10.1%), G. lambelia one case (1.1%), and B. hominis in 5 (5.2%). Blastocystis hominis was significantly prevalent among IBS-all types than the control group (P. value 0.02). It is more frequent among patients with IBS- D than those with IBS-C (P. value 0.001). Conclusion: This study concluded that, there is a statistical association between presence of intestinal parasites and IBS on study population. Blastocystis hominis was found more frequently detected parasite, and it was found significantly associated with IBS- diarrhea.
| Published in | American Journal of Health Research (Volume 4, Issue 4) |
| DOI | 10.11648/j.ajhr.20160404.12 |
| Page(s) | 82-85 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Irritable Bowel Syndrome (IBS), Intestinal Parasites, Blastocystis hominis, Giardia Lamblia
| [1] | Sinha P, Ghoshal UC, Choudhuri G, Naik S, Ayyagari A, Naik SR. Does Entamoeba histolytica cause irritable bowel syndrome? Indian J Gastroenterol 1997; 16 (4): 130- 133. |
| [2] | Wong KH, Ng GC, Lin RT, Yoshikawa H, Taylor MB, Tan KS. Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Parasitol Res 2008; 102 (4): 663-670. |
| [3] | Ramirez-Miranda ME1, Hernandez-Castellanos R, Lopez-Escamilla E, Moncada D, Rodriguez-Magallan A, Pagaza-Melero C, Gonzalez-Angulo A, Flisser A, Kawa- Karasik S, Maravilla P. Parasites in Mexican patients with irritable Bowel syndrome: a case-control study. Parasites Vectors 2010; 3: 96. |
| [4] | Tan KSW. New insights on classification, identification and clinical relevance of Blastocystis spp. Clin Microbiol Rev 2008; 21 (4): 639-665. |
| [5] | Occhipinti K, Smith JW. Irritable Bowel Syndrome: A Review and Update. Clinics in Colon and Rectal Surgery. 2012; 25 (1): 46-52. |
| [6] | Eltayeb LB, Aljafari AS, Brair LS, Algazy SA, Nail AM. Assessment and Evaluation Of The Effect Of Irritable Bowel Syndrome (IBS) On The Health Related Quality Of Life Among Sudanese Patients. Am. J. Life. Sci. Res 2015; 3 (4): 254-459. |
| [7] | OKeke EN, Agaba EI, Gwamzhi L, Achinge GI, Malu AO. Prevalence of irritable bowel syndrome in a Nigerian student population. Afr. J. Med. Med. Sci 2005; 34: 33-36 |
| [8] | Thompson WG, Heaton KW, Smyth GT, Smyth C. Irritable bowel syndrome in general practice: Prevalence, characteristics and referral. Gut. 2000; 46: 78-82. |
| [9] | Levy RL, Jones KR, Whithead WE, Feld SI, Talley NJ, Corey LA. Irritable bowel syndrome in twins: heredity and social learning both contribute to etiology. Gastroenterology 2001; 121: 799-804. |
| [10] | Wang J, Lu S, Zhao S. Post-infectious and non post-infectious irritable bowel syndrome: A comparative study. Pakistan Journal of Medical Sciences. 2016; 32 (1): 116-119. doi: 10.12669/pjms.321.8628. |
| [11] | Rodriguez LA, Ruigomez A. Increased risk of irritable bowel syndrome after bacterial gastroenteritis: cohort study. BMJ 1999; 318, 565-566. |
| [12] | Stark D, vanHal S, Marriott D, Ellis J, Harkness J. Irritable bowel syndrome: a review on the role of intestinal protozoa and the importance of their detection and diagnosis. Int J Parasitol 2007 Jan; 37 (1): 11-20. |
| [13] | Ragavan ND, Kumar S, Chye TT, Mahadeva S, Shiaw-Hooi H. Blastocystis sp. in Irritable Bowel Syndrome (IBS)--Detection in Stool Aspirates during Colonoscopy. PLoS One. 2015 Sep 16; 10 (9): e0121173. doi: 10.1371/journal. pone.0121173. eCollection 2015. |
| [14] | Clarke SC, McIntyre M. Modified detergent Ziehl-Neelsen technique for the staining of Cyclospora cayetanensis. J Clin Pathol 1996; 49 (6): 511-512. |
| [15] | Clark CG, Diamond LS. Methods for cultivation of luminal parasitic protists of clinical importance. Clin Microbiol. Rev 2002 Jul; 15 (3): 329-334. |
| [16] | Longstreth GF, Thompson WG, Chey WD, Houghton LA, Mearin F, Spiller RC. Functional bowel disorders. Gastroenterology 2006; 130 (5): 1480-1491. |
| [17] | Hussain R, Jaferi W, Zuberi S, Baqai R, Abrar N, Ahmed A, Zaman V. Significantly increased IgG2 subclass antibody levels to Blastocystis hominis in patients with irritable bowel syndrome. Am J Trop Med Hyg 1997; 56 (3): 301-306. |
| [18] | Eltayeb LB, Brair SL, Aljafari AS. The impact of intestinal protozoan parasites among Irritable Bowel Syndrome patients in Khartoum state. Al Neelain Medical Journal 2013; 3 (9): 47-57. |
| [19] | Bujanda L, Gutierrez Stampa MA, Caballeros CH, et al. Trastornos gastrointestinales en Guatemala y su relacion con infecciones parasitarias [Gastrointestinal disorders in Guatemala and their relationship with parasitic infections]. Ann Med Interne 2002; 19 (1): 36. |
| [20] | D’Anchino M, Orlando D, De Feudis L. Giardia lamblia infections become clinically evident by eliciting symptoms of irritable bowel syndrome. J Infect 2002; 45 (3): 169-172. |
| [21] | Stark D1, Beebe N, Marriott D, Ellis J, Harkness J. Prospective study of the prevalence, genotyping and clinical relevance of Dientamoeba fragilis infections in an Australian population. J Clin Microbiol 2005; 43 (6): 2718-2723. |
| [22] | Yakoob J, Jafri W, Jafri N, Khan R, Islam M, Beg MA, Zaman V. Irritable bowel syndrome: in search of an etiology: role of Blastocystis hominis. Am J Trop Med Hyg 2004; 70 (4): 383-385. |
| [23] | Tungtrongchitr A, Manatsathit S, Kositchaiwat C, Ongrotchanakun J, Munkong N, Chinabutr P, Leelakusolvong S, Chaicumpa W. Blastocystis hominis infection in irritable bowel syndrome patients. Southeast Asian J Trop Med Public Health 2004; 35 (3): 705-710. |
| [24] | Grazioli B1, Matera G, Laratta C, Schipani G, Guarnieri G, Spiniello E, Imeneo M, Amorosi A, Foca A, Luzza F. Giardia lamblia infection in patients with irritable bowel syndrome and dyspepsia: a prospective study. World J Gastroenterol 2006; 12 (12): 1941-1944. |
| [25] | Corcoran GD, O'Connell B, Gilleece A, Mulvihill TE. Entamoeba coli as possible cause of diarrhea. Lancet 1991; 338 (8761): 254. |
APA Style
Sara Lavinia Brair, Linda Bashier Eltayeb, Sara Abdelghani Elgazy, Abdelsalam Mohammad Nail, Alfatih Saifudinn Aljafari. (2016). Frequency of Intestinal Parasites Among Sudanese Patients with Irritable Bowel Syndrome. American Journal of Health Research, 4(4), 82-85. https://doi.org/10.11648/j.ajhr.20160404.12
ACS Style
Sara Lavinia Brair; Linda Bashier Eltayeb; Sara Abdelghani Elgazy; Abdelsalam Mohammad Nail; Alfatih Saifudinn Aljafari. Frequency of Intestinal Parasites Among Sudanese Patients with Irritable Bowel Syndrome. Am. J. Health Res. 2016, 4(4), 82-85. doi: 10.11648/j.ajhr.20160404.12
AMA Style
Sara Lavinia Brair, Linda Bashier Eltayeb, Sara Abdelghani Elgazy, Abdelsalam Mohammad Nail, Alfatih Saifudinn Aljafari. Frequency of Intestinal Parasites Among Sudanese Patients with Irritable Bowel Syndrome. Am J Health Res. 2016;4(4):82-85. doi: 10.11648/j.ajhr.20160404.12
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Download@article{10.11648/j.ajhr.20160404.12,
author = {Sara Lavinia Brair and Linda Bashier Eltayeb and Sara Abdelghani Elgazy and Abdelsalam Mohammad Nail and Alfatih Saifudinn Aljafari},
title = {Frequency of Intestinal Parasites Among Sudanese Patients with Irritable Bowel Syndrome},
journal = {American Journal of Health Research},
volume = {4},
number = {4},
pages = {82-85},
doi = {10.11648/j.ajhr.20160404.12},
url = {https://doi.org/10.11648/j.ajhr.20160404.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20160404.12},
abstract = {The objective of this case control study is to identify the relationship between intestinal parasitic infection and Irritable Bowel Syndrome (IBS) among Sudanese patients. Method: Two hundred patients diagnosed with irritable bowel syndrome (IBS) and were attended Health Facilities at Khartoum State were enrolled, together with 99 apparently healthy controls. Patients were classified into three groups (IBS-all types, IBS with diarrhea (IBS-D), and IBS with constipation (IBS-C)). Stool specimens were collected form study population and all were subjected to microscopic examination following wet saline preparation, formal-ether concentration technique, Z. N stain, and in vitro cultivation in Locke egg medium. Results: Intestinal parasites were detected in 32% (64/200) of IBS-all types; and in 16.2% (16/99) of control group (P. value 0.005). For patients, 20 (10%) were positive for the Entamoeba cyst, 3 (1.5%) Giardia lamblia cysts, and 41 (20.5%) were positive for Blastocystis hominis cyst. For control, Entamoeba species were detected in 10 (10.1%), G. lambelia one case (1.1%), and B. hominis in 5 (5.2%). Blastocystis hominis was significantly prevalent among IBS-all types than the control group (P. value 0.02). It is more frequent among patients with IBS- D than those with IBS-C (P. value 0.001). Conclusion: This study concluded that, there is a statistical association between presence of intestinal parasites and IBS on study population. Blastocystis hominis was found more frequently detected parasite, and it was found significantly associated with IBS- diarrhea.},
year = {2016}
}
TY - JOUR T1 - Frequency of Intestinal Parasites Among Sudanese Patients with Irritable Bowel Syndrome AU - Sara Lavinia Brair AU - Linda Bashier Eltayeb AU - Sara Abdelghani Elgazy AU - Abdelsalam Mohammad Nail AU - Alfatih Saifudinn Aljafari Y1 - 2016/06/18 PY - 2016 N1 - https://doi.org/10.11648/j.ajhr.20160404.12 DO - 10.11648/j.ajhr.20160404.12 T2 - American Journal of Health Research JF - American Journal of Health Research JO - American Journal of Health Research SP - 82 EP - 85 PB - Science Publishing Group SN - 2330-8796 UR - https://doi.org/10.11648/j.ajhr.20160404.12 AB - The objective of this case control study is to identify the relationship between intestinal parasitic infection and Irritable Bowel Syndrome (IBS) among Sudanese patients. Method: Two hundred patients diagnosed with irritable bowel syndrome (IBS) and were attended Health Facilities at Khartoum State were enrolled, together with 99 apparently healthy controls. Patients were classified into three groups (IBS-all types, IBS with diarrhea (IBS-D), and IBS with constipation (IBS-C)). Stool specimens were collected form study population and all were subjected to microscopic examination following wet saline preparation, formal-ether concentration technique, Z. N stain, and in vitro cultivation in Locke egg medium. Results: Intestinal parasites were detected in 32% (64/200) of IBS-all types; and in 16.2% (16/99) of control group (P. value 0.005). For patients, 20 (10%) were positive for the Entamoeba cyst, 3 (1.5%) Giardia lamblia cysts, and 41 (20.5%) were positive for Blastocystis hominis cyst. For control, Entamoeba species were detected in 10 (10.1%), G. lambelia one case (1.1%), and B. hominis in 5 (5.2%). Blastocystis hominis was significantly prevalent among IBS-all types than the control group (P. value 0.02). It is more frequent among patients with IBS- D than those with IBS-C (P. value 0.001). Conclusion: This study concluded that, there is a statistical association between presence of intestinal parasites and IBS on study population. Blastocystis hominis was found more frequently detected parasite, and it was found significantly associated with IBS- diarrhea. VL - 4 IS - 4 ER -
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