The aim of this paper is isolating rare actinobacteria from new ecological source as corrosive lesion at concrete infrastructure and screening their ability to produce biological products. Ten pure actinobacteria isolates were isolated from corrosive lesions at concrete infrastructures of irrigation channel using cement extract media at different pHs (9- 12) and incubated at 30°C for 7d. All of the isolates produced variable levels of cellulase and lipase, and nine of them displayed variable levels of alkaline protease and amylase products. Only Four isolates produced extracellular alkaline phosphatase in liquid media. The antagonistic activities of these isolates were screened against four pathogenic microorganisms including Gram positive and negative bacterial species and two species of fungi. Only the isolate ROR40 exhibited antagonism activity against Staphylococcus aureus and Escherichia coli , whereas nine isolates showed different degrees of antagonism activities against Microsporum canis and Trichophyton mentagrophyte. The potential isolate (ROR40) to produce extracellular alkaline phosphatase was selected and identified depending on phenotypical, physiological and molecular according to partial sequences of 16S r RNA gene. It had 98% similarity with Pseudonocardia alni 20049 and P. alni 44104. Finally the isolate is named Pseudonocardia sp. ROR40 (Genbank accession no KJ 725072). The isolate produced extracellular alkaline phosphatase in liquid medium at optimum conditions were pH 8.5 of production medium, 37 °C for 4d. in stand incubator. Conclusion: Our study would be the first instance in comprehensive characterization of concert deteriorating actinobacteria for producing commercially valuable primary and secondary metabolites and it may facilitate us to isolate and characterize more bioactive species.
| Published in | American Journal of Life Sciences (Volume 3, Issue 4) |
| DOI | 10.11648/j.ajls.20150304.11 |
| Page(s) | 247-256 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Concrete Corrosive Lesions, Actinobacteria, Secondary Metabolites, Pseudonocardia, Alkaline Phosphatase
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APA Style
Rabab Omran. (2015). Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria. American Journal of Life Sciences, 3(4), 247-256. https://doi.org/10.11648/j.ajls.20150304.11
ACS Style
Rabab Omran. Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria. Am. J. Life Sci. 2015, 3(4), 247-256. doi: 10.11648/j.ajls.20150304.11
AMA Style
Rabab Omran. Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria. Am J Life Sci. 2015;3(4):247-256. doi: 10.11648/j.ajls.20150304.11
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Download@article{10.11648/j.ajls.20150304.11,
author = {Rabab Omran},
title = {Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria},
journal = {American Journal of Life Sciences},
volume = {3},
number = {4},
pages = {247-256},
doi = {10.11648/j.ajls.20150304.11},
url = {https://doi.org/10.11648/j.ajls.20150304.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150304.11},
abstract = {The aim of this paper is isolating rare actinobacteria from new ecological source as corrosive lesion at concrete infrastructure and screening their ability to produce biological products. Ten pure actinobacteria isolates were isolated from corrosive lesions at concrete infrastructures of irrigation channel using cement extract media at different pHs (9- 12) and incubated at 30°C for 7d. All of the isolates produced variable levels of cellulase and lipase, and nine of them displayed variable levels of alkaline protease and amylase products. Only Four isolates produced extracellular alkaline phosphatase in liquid media. The antagonistic activities of these isolates were screened against four pathogenic microorganisms including Gram positive and negative bacterial species and two species of fungi. Only the isolate ROR40 exhibited antagonism activity against Staphylococcus aureus and Escherichia coli , whereas nine isolates showed different degrees of antagonism activities against Microsporum canis and Trichophyton mentagrophyte. The potential isolate (ROR40) to produce extracellular alkaline phosphatase was selected and identified depending on phenotypical, physiological and molecular according to partial sequences of 16S r RNA gene. It had 98% similarity with Pseudonocardia alni 20049 and P. alni 44104. Finally the isolate is named Pseudonocardia sp. ROR40 (Genbank accession no KJ 725072). The isolate produced extracellular alkaline phosphatase in liquid medium at optimum conditions were pH 8.5 of production medium, 37 °C for 4d. in stand incubator. Conclusion: Our study would be the first instance in comprehensive characterization of concert deteriorating actinobacteria for producing commercially valuable primary and secondary metabolites and it may facilitate us to isolate and characterize more bioactive species.},
year = {2015}
}
TY - JOUR T1 - Corrosive Lesions at Concrete Infrastructures as Promising Source for Isolating Bioactive Actinobacteria AU - Rabab Omran Y1 - 2015/06/17 PY - 2015 N1 - https://doi.org/10.11648/j.ajls.20150304.11 DO - 10.11648/j.ajls.20150304.11 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 247 EP - 256 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20150304.11 AB - The aim of this paper is isolating rare actinobacteria from new ecological source as corrosive lesion at concrete infrastructure and screening their ability to produce biological products. Ten pure actinobacteria isolates were isolated from corrosive lesions at concrete infrastructures of irrigation channel using cement extract media at different pHs (9- 12) and incubated at 30°C for 7d. All of the isolates produced variable levels of cellulase and lipase, and nine of them displayed variable levels of alkaline protease and amylase products. Only Four isolates produced extracellular alkaline phosphatase in liquid media. The antagonistic activities of these isolates were screened against four pathogenic microorganisms including Gram positive and negative bacterial species and two species of fungi. Only the isolate ROR40 exhibited antagonism activity against Staphylococcus aureus and Escherichia coli , whereas nine isolates showed different degrees of antagonism activities against Microsporum canis and Trichophyton mentagrophyte. The potential isolate (ROR40) to produce extracellular alkaline phosphatase was selected and identified depending on phenotypical, physiological and molecular according to partial sequences of 16S r RNA gene. It had 98% similarity with Pseudonocardia alni 20049 and P. alni 44104. Finally the isolate is named Pseudonocardia sp. ROR40 (Genbank accession no KJ 725072). The isolate produced extracellular alkaline phosphatase in liquid medium at optimum conditions were pH 8.5 of production medium, 37 °C for 4d. in stand incubator. Conclusion: Our study would be the first instance in comprehensive characterization of concert deteriorating actinobacteria for producing commercially valuable primary and secondary metabolites and it may facilitate us to isolate and characterize more bioactive species. VL - 3 IS - 4 ER -
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