One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants showing root rot and stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups. Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris (teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG of R. solani on faba bean is AG4-HG1.
| Published in | American Journal of Life Sciences (Volume 3, Issue 1) |
| DOI | 10.11648/j.ajls.20150301.19 |
| Page(s) | 47-55 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Anastmosis Group, Rhizoctonia Solani AG4-HGI, PCR, Grouping of isolates, Phylogenetic Analysis
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APA Style
Mohamed Maha Helmy, Gado Emad, El-Deeb Samir, Mostafa Helmy Mostafa. (2015). Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants. American Journal of Life Sciences, 3(1), 47-55. https://doi.org/10.11648/j.ajls.20150301.19
ACS Style
Mohamed Maha Helmy; Gado Emad; El-Deeb Samir; Mostafa Helmy Mostafa. Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants. Am. J. Life Sci. 2015, 3(1), 47-55. doi: 10.11648/j.ajls.20150301.19
AMA Style
Mohamed Maha Helmy, Gado Emad, El-Deeb Samir, Mostafa Helmy Mostafa. Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants. Am J Life Sci. 2015;3(1):47-55. doi: 10.11648/j.ajls.20150301.19
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Download@article{10.11648/j.ajls.20150301.19,
author = {Mohamed Maha Helmy and Gado Emad and El-Deeb Samir and Mostafa Helmy Mostafa},
title = {Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants},
journal = {American Journal of Life Sciences},
volume = {3},
number = {1},
pages = {47-55},
doi = {10.11648/j.ajls.20150301.19},
url = {https://doi.org/10.11648/j.ajls.20150301.19},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.20150301.19},
abstract = {One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants showing root rot and stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups. Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris (teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG of R. solani on faba bean is AG4-HG1.},
year = {2015}
}
TY - JOUR T1 - Phenotypic Diversity and Molecular Identification of the Most Prevalent Anastomosis Group of Rhizoctonia solani Isolated from Diseased Faba Bean Plants AU - Mohamed Maha Helmy AU - Gado Emad AU - El-Deeb Samir AU - Mostafa Helmy Mostafa Y1 - 2015/02/13 PY - 2015 N1 - https://doi.org/10.11648/j.ajls.20150301.19 DO - 10.11648/j.ajls.20150301.19 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 47 EP - 55 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.20150301.19 AB - One hundred and thirty one isolates of Rhizoctonia spp. were isolated from faba bean plants showing root rot and stem canker collected from different fields in Delta region of Egypt. Forty six isolates were found to be polynucleate and the remaining isolates were binucleates. According to morphological features of isolates, they were classified into 12 groups. Polynucleate isolates were identified as Rhizoctonia solani (Kühn). The most aggressive isolate of R. solani was identified according to sequences of ITS1-5.8S rDNA-ITS4 and the sequence was compared with Thanatephorus cucumeris (teleomorphic phase) and other R. solani (NCBI GenBank). Sequence and comparison revealed that this isolate is R. solani AG4-HGI. Anastomosis test carried out between molecular identified isolate and 11 randomly chosen isolates resembles all groups of polynucleate R. solani. All tested isolates were completely fused between each other indicating that the prevalent AG of R. solani on faba bean is AG4-HG1. VL - 3 IS - 1 ER -
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